apc conjugated anti human cd133 1 antibody (Miltenyi Biotec)
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Miltenyi Biotec
apc conjugated anti human cd133 1 antibody
Apc Conjugated Anti Human Cd133 1 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1554 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/apc+anti+human+cd133+1/CD133%2F1+Antibody%2C+anti-human/10__1007_slash_s00018___026___06376___x-166-10-19
Average 95 stars, based on 1554 article reviews
Apc Conjugated Anti Human Cd133 1 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1554 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/apc+anti+human+cd133+1/CD133%2F1+Antibody%2C+anti-human/10__1007_slash_s00018___026___06376___x-166-10-19
Average 95 stars, based on 1554 article reviews
apc conjugated anti human cd133 1 antibody - by Bioz Stars,
2026-10
95/100 stars
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Staining:Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); Suspension:Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); Article Title: Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells into GABAergic Interneurons Article Snippet: hESC-derived GPCs between day 188 and 304 were dissociated for 8 min in Accutase (StemPro, Thermo Fisher, Waltham, MA, USA) and resuspended to 1 million cell/mL in Miltenyi wash buffer (phosphate-buffered saline [PBS, Gibco, Thermo Fisher; Waltham, MA, USA] + 0.5% bovine serum albumin Fraction V [BSA, Gibco, Thermo Fisher, Waltham, MA, USA] + 2 μM EDTA + 0.05‰ Phenol Red [Sigma-Aldrich, St. Louis, MO, USA]). .. A total of 100 μL of cell suspension was aliquoted per flow cytometry tube and incubation with fluorochrome-labeled antibodies (PE anti-human CD140a, 1:10 [BD Biosciences, cat. no. 556002, Eysins, Switzerland]; APC anti-CD44, 1:500 [Miltenyi, cat. no. 130-095-177, Bergisch Gladbach, Germany]; Incubation:Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); Article Title: Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons Article Snippet: Flow cytometry analysis and FACS sorting For flow cytometry analysis of the cells between D130-270, the cell clusters were dissociated for 8 min in Accutase (StemPro; Thermo Fisher) and resuspended to 1 million cell/ml in Miltenyi wash buffer (PBS + 0.5% BSA Fraction V + 2 μM EDTA + 0.05‰ Phenol Red). .. For each staining, 100 ul cell suspension was used and the cells were incubated with fluorophore-conjugated antibodies for 15 min at 4°C (PE anti-human CD140a, BD Biosciences, cat. no. 556002, 1:10; APC anti-CD44 (Miltenyi, cat. no. 130-095-177, 1:500); Article Title: Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells into GABAergic Interneurons Article Snippet: hESC-derived GPCs between day 188 and 304 were dissociated for 8 min in Accutase (StemPro, Thermo Fisher, Waltham, MA, USA) and resuspended to 1 million cell/mL in Miltenyi wash buffer (phosphate-buffered saline [PBS, Gibco, Thermo Fisher; Waltham, MA, USA] + 0.5% bovine serum albumin Fraction V [BSA, Gibco, Thermo Fisher, Waltham, MA, USA] + 2 μM EDTA + 0.05‰ Phenol Red [Sigma-Aldrich, St. Louis, MO, USA]). .. A total of 100 μL of cell suspension was aliquoted per flow cytometry tube and incubation with fluorochrome-labeled antibodies (PE anti-human CD140a, 1:10 [BD Biosciences, cat. no. 556002, Eysins, Switzerland]; APC anti-CD44, 1:500 [Miltenyi, cat. no. 130-095-177, Bergisch Gladbach, Germany]; Article Title: A distinct lineage pathway drives parvalbumin chandelier cell fate in human interneuron reprogramming Article Snippet: Briefly, cells were mechanically detached and dissociated with Accutase (StemPro, Thermo Fisher Scientific; Waltham, MA, USA) for 8 min and were resuspended in Miltenyi wash buffer [phosphate-buffered saline (PBS), Gibco, Thermo Fisher Scientific; Waltham, MA, USA; 0.5% BSA Fraction V Gibco, Thermo Fisher Scientific; Waltham, MA, USA; 2 μM EDTA; and 0.05‰ (per mil) Phenol Red, Sigma-Aldrich, St. Louis, MO, USA] in a concentration of 1 × 10 6 cells/ml. .. This was followed by incubation with fluorochrome-conjugated antibodies [PE anti-human CD140a (BD Biosciences, catalog no. 556002, Eysins, Switzerland 1:10), APC anti-CD44 (Miltenyi, catalog no. 130-113-331, Bergisch Gladbach, Germany, 1:100), Article Title: Generating human parvalbumin interneurons through 3D glia reprogramming Article Snippet: Briefly, cells were mechanically detached and dissociated with Accutase (StemPro, Thermo Fisher; Waltham, MA, USA) for 8 min and were resuspended in Miltenyi wash buffer (PBS, Gibco, Thermo Fisher; Waltham, MA, USA; 0.5% BSA Fraction V Gibco, Thermo Fisher; Waltham, MA, USA; 2μM EDTA; 0.05‰ Phenol Red Sigma-Aldrich, St. Louis, MO, USA) in a concentration of 1×10 cells/ml. .. This was followed by incubation with fluorochrome-conjugated antibodies (PE anti-human CD140a, [BD Biosciences, cat. no. 556002, Eysins, Switzerland 1:10]; APC anti-CD44, [Miltenyi, cat. no. 130-113-331, Bergisch Gladbach, Germany, 1:100]; Flow Cytometry:Article Title: Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells into GABAergic Interneurons Article Snippet: hESC-derived GPCs between day 188 and 304 were dissociated for 8 min in Accutase (StemPro, Thermo Fisher, Waltham, MA, USA) and resuspended to 1 million cell/mL in Miltenyi wash buffer (phosphate-buffered saline [PBS, Gibco, Thermo Fisher; Waltham, MA, USA] + 0.5% bovine serum albumin Fraction V [BSA, Gibco, Thermo Fisher, Waltham, MA, USA] + 2 μM EDTA + 0.05‰ Phenol Red [Sigma-Aldrich, St. Louis, MO, USA]). .. A total of 100 μL of cell suspension was aliquoted per flow cytometry tube and incubation with fluorochrome-labeled antibodies (PE anti-human CD140a, 1:10 [BD Biosciences, cat. no. 556002, Eysins, Switzerland]; APC anti-CD44, 1:500 [Miltenyi, cat. no. 130-095-177, Bergisch Gladbach, Germany]; |